Jerry Doyle (Michael Garibaldi) and Mira Furlan (Delenn) are the only actors to appear in both the first pilot ("The Gathering") and the series finale ("Sleeping in Light") whereas Doyle, Furlan, Claudia Christian (Susan Ivanova) and Stephen Furst (Vir Cotto) are the only actors to appear in both the second pilot ("Midnight on the Firing Line") and the series finale.

Vir: You know, Londo never really liked the Pak'ma'ra. I mean…they're stubborn, lazy, obnoxious, greedy…
Garibaldi: And kinda look like an octopus that got run over by a truck.
Vir: That, too. But…one time, we were walking through the Alien sector, and we heard this beautiful singing coming from the Pak'Ma'Ra's quarters.
Sheridan: They can sing?
Stephen: There's nothing in the literature about that.
Vir: Apparently, they only ever do it once a year, during their religious period. And we were listening to this singing and I saw a tear run down Londo's face, and I said, "We should go, this is upsetting you." But he said no, and we stayed. After the singing was over he turned to me and said, "There are 49 Gods in our Pantheon, Vir. To tell you the truth I've never really believed in any of them. But if just one of them exists…then God sings with that voice."

Sheridan: A toast. To…absent friends. In memory still bright.
Garibaldi: G'Kar.
Vir: Londo.
Delenn: Lennier.
Franklin: Mar--
Ivanova: Marcus.

(As he nears the end of his life, Sheridan returns to Babylon 5 which is soon to be decommissioned)
Zack: So you hear 'em too, huh?
Sheridan: Zack. What the hell are you doing here? I thought you went back to Earth.
Zack: Yeah, I did. Got bored. Re-upped about six months ago. I figured I'd be here 'til they turn the lights out.

Sheridan: Well…look at that…the sun's coming up...

Ivanova: (narrating; last lines of the series) Babylon 5 was the last of the Babylon stations. There would never be another. It changed the future…and it changed us. It taught us that we have to create the future or others would do it for us. It showed us that we have to care for one another, because if we don't, who will? And that true strength sometimes comes from the most…unlikely places. Mostly though, I think it gave us hope, that there can always be new beginnings…even for people like us. As for Delenn, every morning for as long as she lived, Delenn got up before dawn and watched the sun come up.