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Several Arkham inmates, including the Scarecrow, Ventriloquist and Harley Quinn lodge complaints against Lyle Bolton, the Asylum's sadistic new chief of security. When Bolton is fired, he vows revenge on societies' true lunatics, the officials whose policies have turned Gotham City into a haven for crime. Taking on the new identity of master jailer Lock-Up, Bolton kidnaps several prominent Gothamites, including Commissioner Gordon and Mayor Hill, and locks them away on an abandoned prison ship. It's up to Batman and Robin to confront Lock-Up and battle him for the lives of those he's wrongfully imprisoned.
Final appearance of Scarecrow.
Lock-Up was another villain created specifically for the show, much like Harley Quinn
Bullock: Just what this town needs, another psycho in a Halloween costume
Robin: Another fine villain brought to via a grant from the Wayne Foundation.
Bartholomew: It's all right. I promise, you can speak freely here.
(Bolton sneers at Ventriloquist and Scarface)
Ventriloquist: Mr. Bolton? He's a..
Scarface: A hard worker! A real stand-up guy. No complaints here, chief.
Bartholomew: Ms. Quinn? Do you have any complaints against Mr. Bolton?
(Lyle Bolton sneers threateningly at Harley)
Harley Quinn: I got nothing to say.
Bartholomew: But surely you must have something you wish to..
Harley Quinn: You got a hearing problem or something?! NO! NADA! IXNAY! ZERO! ZILCH! BUPKES! NOTHING! OKAY?!
Robin: Nice look...early Alcatraz.
Lyle Bolton: Congratulations, Professor Crane, you're the first inmate to escape Arkham under my charge. You're also the last.
Lyle Bolton: This city is an open wound begging to be stitched.
Robin: That Bolton is a 10 on a creepameter.
Lock-Up: We could have made our own order!!
Batman: I was born to fight your brand of order!