This episode is a rossover between CSI and Two & Half Men. The first half "Two and a Half Deaths" was written by the writers of CSI, followed by the second half hour written by the writers of Two and Half Men.

Hodges: Bud didn't marry Annabelle. Bud married Natasha pretending to be Annabelle.
Catherine: Well, one thing's for sure. Neither one of them is pretending to be dead.

Bud: Annabelle and I married two days ago.
Spencer: Oh, I don't believe that.
Bud: Oh, no it's true. We had a deal. She said that if I gave up coke that we could get married.
Spencer: But you never gave up coke.
Bud: She didn't know that.

Henry: So, the clot doesn't thicken but the plot does.

David: Sorry for the delay, it's a pappazzi jungle downstairs.
Grissom: Yeah, well Doc Robbins isn't the only one with a scrap book.

Warrick: Hey, what does this look like to you?
Grissom: Hermaphrodite on rollerskates.
Catherine: A puppy.

Bud: Whoa hey! Easy on the Emmy there buddy.
Grissom: Is that what this is?

Greg: So, that's it. Robot-man, closet-stalker-guy gets his package grabbed and a TV star dies.
Nick: It is the classic story.

Lytle: Beautiful people doing high-tech police work. There might be a series in this.

Grissom: Did she also have a drug problem?
Lytle: Define problem.
Grissom: The kind that requires using other people's urine to past a drug test.
Lytle: Then yes.

Spencer: Sure, I created the show. When the show became a hit, she became a star. I became a highly paid butt-monkey.
Brass: Well, it's a skill like anything else.

Grissom holds up an iPod touch to show a clip to one of the suspects. In the scene, the iPod plays sound, but iPod touch doesn't have any external speaker.

The rug that Megan Kupowski kneels on when mourning her dead dog changes positions between shots during the scene.

Title: Two and a Half Deaths

The episode title is a take on the CBS sitcom title Two and a Half Men. The episode title reflects the fact that the episode is a cross-over with the popular CBS sitcom, with writers from both shows sharing duties. CBS did a number of crossover interactions in its series the week of the broadcast. During the episode, the stars of Two and a Half Men are also seen standing outside a studio trailer.

Bud: Whoa hey! Easy on the Emmy there buddy.
Grissom: Is that what this is?

The Emmy award is usually considered to be TV's highest award, equivalent to a movie Oscar. Grissom and Bud's exchange is a running gag for both CSI and Two and a Half Men. Both shows have received multiple Emmy nominations over the course of their long runs, but neither have won an Emmy.

Brass: (to Grissom) Forget it, Gil. It's Burbank.

Brass' line is a reference to an infamous quip from the film Chinatown: "Forget it, Jake. It's Chinatown."

Henry: What we have here is failure to coagulate.

Henry's line is a reference to a classic line in the 1967 film, Cool Hand Luke: "What we have here is failure to communicate."

Nick: A mime is a terrible thing to waste.

Nick is referencing the slogan of the United Negro College Fund: "A mind is a terrible thing to waste."

Grissom: Dying is easy, comedy is hard.

Grissom is slightly misquoting the last words of Oscar winning actor Edmund Gwenn: "Dying is easy. Comedy is difficult." Gwenn won his Oscar as a supporting actor in the classic film, Miracle on 34th Street, playing Santa Claus.

Frieberg: She hates everything and I'm afraid of vaginas.
Mark: I'm only afraid of the ones with teeth.

In the 2007 film Teeth, young women have teeth lining their vaginas. The term, however, long predates that movie, generally referencing a bad sort of woman that you wind up regretting getting involved with.