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After a pregnancy scare, Lois convinces Peter to get a vasectomy. Just in case they wanted to have more children, Peter goes to a sperm bank to preserve his sperm. But in a mishap, Peter breaks all the jars and fills them up with his own sperm. Thus, creating Stewie's half-brother and rival Bertram when a lesbian couple decide they want a baby. Meanwhile, Stewie takes his place as king of the playground but when Betram shows up he must defend his terf.
Betram, Stewie's sperm brother, returns in this episode for the first time since "Emission Impossible".
One of the lesbians has also been seen before as Meg's gym teacher.
Brian: Lois has gained some weight since you guys stopped having sex. Maybe you should, uh, you know, have some sex.
Lois: Peter I'm not hungry.
Peter: I want you bigger, I want you fatter, it will please me.
Chris: Hey Doc? What did you do with my Mom's fat?
Doctor: Oh it's right here in this storage closet.
(He opens the closet door to find Peter with his pants down sucking on Lois's fat)
Peter: Uh, it's exactly what it looks like...
Peter: (sees fat Lois come in) Oh, hey Hogzilla.
Peter: Boy, fat sex is the best sex we've ever had! Last night there were so many boobs I didn't know who's boobs I was grabbin'!
Stewie: Anyone else want to feel my Reebok in their grapes? Then you'll all do as I say!
Cleveland: Maybe you should freeze some of your sperm.
Peter: I don't know Cleveland, that didn't work out that time I tried to freeze my nuts.
(cutaway to the squirrell from "Ice Age" trying to pry an acorn from the ice. Shows Peter)
Peter: NO! NO! Those are MY NUTS! MY NUTS! Awww, you're just a hungry little fella aren't ya? But those are MY NUTS!
(the squirrell attacks Peter off-screen)
Peter: OW MY NUTS!!!
Obviously, it would be nearly impossible for Peter to fill all the Sperm containers by himself in the time he was in the room.
Of course, if Betram's sperm cell had been transferred into another woman's body, he would not look the same as he did in Emission Impossible; since the egg cell in the lesbian lady would have different genetics than Lois.
Bertram: I feel a presence i've not felt since...
These are the exact lines spoken by Darth Vader in the original Star Wars movie when Obi-Wan Kanobi returns.
Peter: Blanka, Zangief, Chun-Li, Guile, Honda...
This is a direct and obvious reference to the Capcom's Street Fighter video game series.
Stewie: At my signal, unleash hell.
These are the exact words spoken by Maximus Decimus Meridius before the battle in the opening scene of the year 2000 film Gladiator.
Stewie: You got my Cheez Wiz, boy?
This is a direct quote taken from the Blues Brothers movie. When Elwood Blues goes back to his hotel, a man asks him that exact question and Elwood throws him back the Cheez Wiz just like the boy does to Stewie in this episode.
The scene where Peter is attacked by a squirrel after defending his nuts; that scene is actually an exact promo scene taken from the movie Ice Age (minus the Peter part). It would be my guess that FOX asked them to add this, since the squirrel (Scat) from the movie had been hosting the "Animation Domination" that night.
When Stewie and Brian try to "Find diversity in a Abercrombie and Fitch catalog", a black "Where's Waldo" can be seen in the book.
The entire "Stewie vs Betram" thing is based on an earlier episode, "Emission Impossible". In which Stewie and Betram fought as sperm in Peter's body. This is Bertrams first appearance since then.