"Twice Shy" was filmed out of order, before "Terra Firma," to allow production time to prepare the sets necessary for that episode.
There are numerous references in this episode to the second season's "Beware of Dog," in which Chiana brought another alien creature on board which turned out to be a major problem for the crew.
To prepare for her debut directorial stint on Farscape, Kate Woods immersed herself in the series, watching over thirty episodes from the preceding three seasons.
The producers had wanted to cast noted Australian actress Paula Arundell for some time, and had previously considered her for the role of Neeyala in the Season 3 two parter "Wait for the Wheel."
Guy Gross deliberately scored the scenes featuring Talikaa with an unusual combination of sounds, including a dulcimer, more usually associated with Elizabethan music.
Since the spider was created entirely by the CG department at Animal Logic, the actors had to work with a large silver ball on set to give them an idea of where the creature would be.
Dave Elsey designed the potential version of the Talikaa Spider, which would have been half woman and half spider, similar to the creatures created by Ray Harryhausen (Jason and the Argonauts).
The disease spread by the spider was originally far more graphically displayed, with giant goiters on the victims' necks, and pieces of latex twisting the skin, inspired by the make up in the film The Incredible Melting Man. In the end, this was toned down.
The scenes showing the victims of the disease had to be filmed on four separate days, although in the end only short scenes were used in the final episode.
The tag scene between Crichton and Aeryn was considerably expanded and developed on set to allow Aeryn a chance to vent her feelings.