"Man Up" is about three men who try to reignite the tough guy in them and what it takes to be the "real man" as Will (Mather Zickel), who is married to Theresa (Teri Polo), wants to find out what it takes to bring out the "real man" inside. Being a man in these times has changed and Will is focused only on getting his son Nathan (Jake Johnson) the perfect gift for his 13th birthday. While Craig (Christopher Moynihan) still has feelings for his college ex-girlfriend Lisa, Kenny (Dan Fogler) suppresses his anger towards his ex-wife Brenda (Amanda Detmer) by saying, "What would Tobey Maguire do? When Brenda starts dating a new guy (Henry Simmons), Kenny has to try even harder.
Craig crashes Lisa's wedding and tries to win her back by singing to her, the groom and his best man race after them to see if they will "Man Up" and take care of their own. Created by Christopher Moynihan (100 Questions) "Man Up" answers the question, "What does it take today to be a man's man?" and is filled with non-stop comedy with the help of the other cast members.
Will hopes that adopting a baby chick for little Lucy will be a "teachable moment" about where food comes from, but things get out of hand when all of the guys decide they want to adopt chicks too; Craig dates an attractive doctor, but finds himself intimidated by her bully of a son.