We learn that Dean is 27 years old.

Dean: Have you ever watched daytime TV? It's terrible.
Sam: I talked to your doctor.
Dean: That fabric softener teddy bear. Oooh! I'm gonna hunt that little bitch down.
Sam: Dean.
Dean: Yeah, all right, well, it looks like you're going to leave town without me.
Sam: What are you talking about? I'm not going to leave you here.
Dean: Hey, you better take care of that car, or I swear I'll haunt your ass.
Sam: I don't think that's funny.
Dean: Ah, come on. It's a little funny. Look, Sammy, what can I say, man? It's a dangerous gig. I drew the short straw. That's it. end of story.
Sam: Don't talk like that, all right? We still have options.
Dean: What options? You got burial or cremation. I know it's not easy, but I'm gonna die and you can't stop it.
Sam: Watch me.

Dean: I'm not gonna die in a hospital where the nurses aren't even hot.

Sam: You know this whole "I laugh in the face of death" thing is crap, I can see right through it.
Dean: Yeah, whatever. Have you even slept? You look worse than me.

Dean: You're not gonna let me die in peace, are you?
Sam: I'm not gonna let you die, period.

Dean: (arriving at a faith healer) Man, you're a lying bastard. I thought you said we were going to see a doctor.
Sam: I believe I said a specialist.

David Wright: Roy Le Grange is a fraud. He's no healer.
Dean: Amen, brother.
Sam: You keep up the good work.

Dean: And God save us from half the people who think they're doing God's work.

Dean: It must be rough, to believe in something so much and have it disappoint you like that.
Layla Rourke: You wanna hear something weird? I'm okay, really. I guess if you're going to have faith you can't just have it when the miracles happen. You have to have it when they don't.
Dean: So what now?
Dean: Hey, um, you know, I'm not much of a praying type. But I'm gonna pray for you.
Layla Rourke: Well, there's a miracle right there.

Dean: You ever actually watch daytime TV? It's terrible.

This is a joke about Jensen Ackles (Dean) starring as Eric Brady for several years on the daytime soap Days of our Lives.

Dean: That fabric softener teddy bear. Oooh! I'm gonna hunt that little bitch down.

Dean is referring to the Snuggle bear on the Snuggle brand of fabric softener commericals.

Grim Reaper

In various religions on Earth, the Grim Reaper is seen as the Angel of Death that carries the souls of the recently departed into their afterlife.

In literature, the Grim Reaper is often shown wearing a dark cloak and carrying a scythe. It become a figure of death in several stories, movies, and television shows and its presence often means that a character will die, similar to this episode. However, traditionally the Grim Reaper only carries away the recently deceased souls, not take their life from them as seen in this episode.