Unused scenes include:

1) Episode 503, Scene 9: As Dean and Sam work at the hotel, they discover that the two victims arrived in town six months ago at the same time, and only had PO Boxes for addresses. Dean is eager to track them down and kill them, while Sam discovers that there's no increase in missing persons. Sam suggests that Dean might be overreacting after the death of their father, and Dean tells him to try enjoying his work.

Sam: (to Dean) Give you a couple of severed heads and a pile of dead cows and you're Mr. Sunshine.

Dean: (talking about the Impala) Woo... listen to her purr. Have you ever heard anything so sweet?
Sam: You know, if you two want to get a room, just let me know, Dean.
Dean: Aww, don't listen to him baby, he doesn't understand us.

Sheriff: What newspaper did you say you worked for?
Dean: World Weekly News.
Sam: Weekly World News.
Dean: World ...
Sam: Weekly World News.
Dean: Wor -- I'm new.
Sheriff: Get out of my office.

Dean: (in a morgue) All right, Open it.
Sam: You open it.
Dean: Wuss.

Sam: Dean, get me a bucket.
Dean: Find something?
Sam: No, I'm going to puke.

Dean: Wait, lift her lip up again.
Sam: What? You want me to throw up, is that it?
Dean: No, no, no. I think I saw something. What is that, a hole?
Sam: It’s a tooth.
Dean: Sam, that’s a fang. A retractable set of vampire fangs, you gotta be kidding me.
Sam: Well, this changes things.
Dean: You think?

Sam: So... we're looking for some people.
Bartender: Sure, it's hard to be lonely.

Dean: Did you check out that Barker farm?
Gordon: It’s a bust. Just a bunch of hippy freaks. Though they could kill you with that patchouli smell alone.

Dean: You all right, Sammy?
Sam: Yeah, I'm fine.
Gordon: Well, lighten up a little, Sammy.
Sam: He's the only one who gets to call me that.

Sam: Look, I'm not going to bring you guys down. I'm just gonna go back to the motel.
Dean: You sure?
Sam: Yeah.
Dean: Sammy! Remind me to beat that buzzkill out of you later, all right?

Dean: So I pick up this crossbow and I hit that ugly sucker with a silver-tipped arrow right in the heart. Sammy's waiting in the car, and me and my dad take that thing into the woods and burn it to a crisp. I'm sitting there and I'm looking into the fire and I'm thinking to myself "I'm sixteen years old, kids my age worried about pimples, prom dates and I'm seeing things that they'll never even know, never even dream of." So right then I just sort of...
Gordon Walker: Embraced the life.
Dean: Yeah.
Gordon Walker: Yeah.
Dean: Yeah.

Dean: He was one of those guys. Took some terrible beatings ... just kept coming. So you're always thinking to yourself, he's indestructible. He'll always be around, nothing can kill my dad. And just like that ... he's gone. Can't talk about this to Sammy ... got to keep my game face on. But, uh... the truth is I'm not handling it very well.

Sam: I thought you said he was a good hunter.
Ellen Harvelle: And Hannibal Lecter's a good psychiatrist.

Gordon Walker: You know why I love this life?
Dean: Hmm?
Gordon Walker: It's all black and white. There's no maybe. You find the bad thing, kill it. Most people spend their lives in shades of gray. "Is this right, is that wrong?" Not us.

Lenore: Survival. No deaths, no missing locals. No reason for people like you to come looking for people like us. We blend in. Our kind is practically extinct. Turns out we weren't quite as high up the food chain as we’d imagined.

Eli: Why are we explaining ourselves to this killer?
Lenore: Eli...
Eli: We choke on cow's blood so that none of them suffer. have to suffer. And tonight, they murdered Conrad, and they celebrated.
Lenore: Eli, that's enough.
Sam: Yeah, Eli, that's enough!

Dean: What part of "vampires" don’t you understand, Sam? If it’s supernatural, we kill it. End of story. That’s our job.
Sam: No, Dean, that is not our job. Our job is hunting evil. And if these things aren’t killing people, they’re not evil.

Dean: A wooden bridge. That's all you got?
Sam: The bridge was four and a half minutes from their farm
Dean: How do you know?
Sam: I counted. They took a left out of the farm, then turned right onto a dirt road, followed that for two minutes, slightly uphill. Then took another quick right and we hit the bridge.
Dean: You're good. You're a monster pain in the ass... but you're good.

Gordon Walker: Sam, Dean, come on in.
Dean: Hey, Gordon, what’s going on?
Gordon Walker: Just poisoning Lenore here with some dead man’s blood. She’s gonna tell us where all her little friends are, aren't you. Want to help?
Dean: Look, man…
Gordon Walker: Grab a knife, I was just about to start in on the fingers.

Dean: I might be like you, and I might not. But you’re the one tied up right now.

Dean: Clock me one.
Sam: What?
Dean: Come on, hit me. I won't even hit you back. Let's go.
Sam: No.
Dean: Let's go, you get a freebie. Come on, hit me!
Sam: You look like you just went twelve rounds with a block of cement, Dean. I'll take a raincheck.

Sam: After what happened to Mom, Dad did the best he could.
Dean: I know he did, but the man wasn’t perfect. And the way he raised us, to hate those things and, man, I hate ‘em. I do. When I killed that vampire at the mill I didn’t even think about it. Hell, I even enjoyed it.
Sam: You didn’t kill Lenore.
Dean: No, but every instinct told me to. I was going to kill her. I was going to kill them all.
Sam: Yeah, Dean, but you didn't. And that’s what matters.

Sam and Dean visit a hospital named Candler County Hospital while in Montana. However, there is no Candler County in Montana.

Employees at the hospital have North Montana written on their badges. The episode is said to take place in Red Lodge, which is in southern Montana, not northern.

After beheading the vampire, blood splatters on Dean's face. In the next shot, the amount of blood has instantly increased and the pattern is different.

As the brothers backtrack Sam's trail, the Impala passes by the camera. The person driving is definitely not Sam or Dean.

When Sam approaches Gordon in the vampire lair, his arms at his sides. when Gordon lunges forward, Sam's hands instantly leap up as if he were surrendering.

When Gordon throws Dean into the next room, Dean falls on a table and half of it sticks up on the floor. In the next shot and all following ones, it disappears entirely as Gordon walks through the space where it was.

Ellen: And Hannibal Lecter is a good psychiatrist.

Hannibal Lecter is a character from the movies Silence of the Lambs, Red Dragon, and Hannibal. Lecter was a psychiatrist before he was caught and convicted of murdering and eating his victims.

Gordon: Easy there, Chachi!

Chachi is Scott Baio's character from the television series Happy Days and Joanie Loves Chachi.

Gordon: Another one bites the dust!

"Another One Bites The Dust" is the title of a 1980 hit song by Queen.

Dean: Put the lotion in the basket.

Dean is quoting a line from the movie The Silence of the Lambs. In the movie, Buffalo Bill, a serial killer who kidnapped women for their skin, would have his victims rub lotion all over themselves to keep their skin moist while trapped in a pit. He would lower the lotion down in a basket and order his victim to put it back into the basket so he could pull it up.

Sheriff: What newspaper did you say you worked for?
Sam: Weekly World News.

Weekly World News is a common supermarket tabloid that reports on supernatural or paranormal findings, most of which are fictional.

Title:

Cleverly, the title doesn't refer to the vampires, but to Gordon Walker.

Deleted Scene Issues:

It's unfortunate that the scene mention was delete, because it leaves two minor plot holes. For one, it explains why Sam is so pensive when they discover that Gordon is killing vampires. For another, it accounts for Dean's statement later in the episode that he told Sam to enjoy himself. Both of those can be found in the unused scene.