Short 1: Batman only appears.
Short 2: Batgirl only appears.
Short 3: Batman and Robin appear.
Villains: 1:Poison Ivy and Harley Quinn. 2:Clayface. 3:Joker.
On the original airing of the Justice League Unlimited episode, Comfort and Joy, this episode aired after it.
This episode was clearly aired out of order, as it features Tim Drake as Robin (who is introduced in "Sins of the Father"), as well as Clayface (whose survival following the events of "Mudslide" from Batman: The Animated Series was not explained until "Growing Pains").