The Brotherhood of Evil and it's allies are all frozen
Dr. Light is the only villian not frozen
Jynx is know on the side of good.
The Titans vs. Doctor Light:
The final scene of the episode is an indirect homage to one of the more recent TT comic book storylines, 'Twight light of the Titans'. In climax of the storyline, Doctor Light faces off againts partically every Titan, past and present.