The Mysterious Cities of Gold was an animated television series co-produced by DiC Entertainment and Studio Pierrot first shown in 1982. The series comprises 39 episodes told in a single continuous narrative.

The story is set in 1532. A young Spanish boy named Esteban joins a voyage to the New World in search of the lost Cities of Gold. He hopes to find his father, from whom he was separated on being rescued from a sinking ship by Magellan's expedition. On arrival in South America Esteban and his companions begin uncovering evidence relating to the Cities of Gold and various ancient technologies, and also become deeply embroiled in a conflict between the Spanish, the various native populations and later, a strange race called the Olmecs.

In the US, it aired on Nick Jr from 1987 to 1990.