| Name (Sort) | Character (Sort) | |
| •Aaron Karo | played | Himself |
| •Alan Thicke | played | Hot Moms of the '80s Host |
| •Allison Munn | played | Herself |
| •Angie Everhart | played | Herself |
| •Antigone Rising | played | Themselves |
| •Bil Dwyer | played | Himself |
| •Biz Markie | played | Himself |
| •Brad Sherwood | played | Himself |
| •Cassandra Peterson | played | Biggest Boobs of the '80s Host (as Elvira) |
| •Chris Booker (1) | played | Himself |
| •Chris Coppola | played | Himself |
| •Chris Wylde | played | Himself |
| •Chuck Woolery | played | Best On-Screen Hookup Host |
| •Dan Frischman | played | Himself |
| •Darryl McDaniels | played | Himself |
| •Debbie Matenopoulos | played | Herself |
| •Edwin McCain | played | Himself |
| •Elon Gold | played | Himself |
| •Emmanuel Lewis | played | Pop Culture Term Host |
| •French Stewart | played | Himself |
| •Genevieve Gorder | played | Herself |
| •Gilbert Gottfried | played | Himself |
| •Godfrey | played | Himself |
| •Greg Fitzsimmons | played | Himself |
| •Hal Sparks | played | Himself |
| •Harry Anderson | played | Guilty/Not Guilty Host |
| •Jake Fogelnest | played | Himself |
| •Jane Wiedlin | played | Herself |
| •Jason Winston George | played | Himself |
| •Jill Whelan | played | Herself |
| •Jo Koy | played | Himself |
| •Joe Lorge | played | Himself |
| •Joel Stein | played | Himself |
| •John Moschitta, Jr. | played | Year in Review Host |
| •Justin Long | played | Himself |
| •Kathleen Madigan | played | Herself |
| •Kevin Weisman | played | Himself |
| •Larry Joe Campbell (1) | played | Himself |
| •Lauren Weedman | played | Herself |
| •Loni Love | played | Herself |
| •Luis Guzman | played | Himself |
| •Mario López | played | Himself |
| •Mark Hoppus | played | Himself |
| •Michael Ian Black | played | Himself |
| •Mo Rocca | played | Himself |
| •Modern Humorist | played | Themselves |
| •Molly Culver | played | Herself |
| •Nicole Eggert | played | Herself |
| •Nicole Sullivan | played | Herself |
| •Patrice O'Neal | played | Himself |
| •Paula Abdul | played | Teen Idols of the '80s Host |
| •Rachael Harris | played | Herself |
| •Rachel Quaintance | played | Herself |
| •Rich Eisen | played | Himself |
| •Sherri Shepherd | played | Herself |
| •Simon Doonan | played | Himself |
| •Stephen Lynch | played | Himself |
| •The Donnas | played | Themselves |
| •Weird Al Yankovic | played | Movies That Should've Been Made in 3D Host |
| •Wil Wheaton | played | Himself |
| •Willie Garson | played | Himself |