| Name (Sort) | Character (Sort) | |
| •Drake Bell | voiced | Peter Parker aka ‘Spider-Man’ (65Eps.) |
| •Ogie Banks | voiced | Luke Cage aka ‘Power Man’ (43Eps.) |
| •Greg Cipes | voiced | Daniel "Danny" Rand aka ‘Iron Fist’ (41Eps.) |
| •Clark Gregg | voiced | Agent Phil Coulson (24Eps.) |
| •Tom Kenny | voiced | Otto Octavius aka ‘Doctor Octopus’ (17Eps.) |
| •Matt Lanter | voiced | Harry Osborn | Eugene "Flash" Thompson (20Eps.) |
| •Misty Lee | voiced | Aunt May Parker (19Eps.) |
| •Stan Lee | voiced | Stan the Janitor (10Eps.) |
| •Caitlyn Taylor Love | voiced | Ava Ayala aka ‘White Tiger’ (41Eps.) |
| •Chi McBride | voiced | Nick Fury (38Eps.) |
| •Logan Miller | voiced | Sam Alexander aka ‘Nova’ (41Eps.) |
| •J.K. Simmons | voiced | J. Jonah Jameson (25Eps.) |
| •Tara Strong | voiced | Mary Jane Watson (17Eps.) |
| •Steven Weber | voiced | Norman Osborn (13Eps.) |